P-114: Effect of In Situ Cool Storage on Goat Epididymal Sperm

Background: Short time preservation of semen is common in animals (e.g. horse or pig etc…) which their sperm in known as freezing sensitive sperm. It also may use for short transportation of other species to be cryopreserved or use in AI or IVF programs. The aim of the present study was to evaluate effects of duration of cool storage on goat epididymal sperm. Materials and Methods: Total numbers of 40 testes epididymes of slaughtered bucks were transported in an ice container to the Lab and submitted to 4 time of storage (0, 24, 48 and 72 hours) in 5°C refrigerator. After storage caudal epididymes were dissected and cut with a stab incision and incubated in sperm collection medium (TALP). Sperm parameters were analyzed after time of storage with focus on sperm abnormalities. GLM procedure was used for data analysis in SAS. Results: The percentages of live sperms were significantly reduced after 48 and 72 hours of cool storage (94.08, 90.08, 76.4 and 79.6 for 0, 24, 48 and 72 hoursrs, respectively p<.0.01). An increasing trend of sperm head abnormalities was detected up to 48 hours (1.7%) of cool storage (p<0.01). Significant increase in tail abnormalities were recorded after 48 and 72 hours of storage (p<0.01). Cytoplasmic droplet significantly decreased after 48 and 72 hours of cool storage (p<0.001). Conclusion: In conclusion, goat epididymal sperm can be preserved in situ in cool enviromnet up to 24 hours. The results showed impact of duration of cool storage on sperm abnormalities of goat epididymal sperm.